To rule out whether hAFSC alone can cause damage to the kidney and/or produce cytokines as soon as injected in vivo, we injected them into nu/nu mice with out ATN induction. In these mice, injected solely with hAFSC there was no alteration of creatinine or BUN, no improve in apoptosis or proliferation or tubular injury; thus indicating that hAFSC per se don’t alter the conventional physiology and morphology of the kidney. In addition, in these mice, there was a rise of ranges in IL-10 and IL-1Ra and IL-6 following hAFSC injection , there was no enhance in SDF-1 and in addition they expressed decrease levels of NK attractors as nicely as macrophage attractors. Thus, hAFSC do stimulate a modest tissue response by nu/nu mice, however this didn’t cause any measurable perturbation of kidney function. In addition, the human cytokines produced by the injected cells in mice that did not bear ATN disappear rapidly and with no obvious antagonistic consequences. In particular, in Figure 6A–C an increase in the number of damaged tubules is seen from 24 hours to seventy two hours in the glycerol-treated control animals.
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